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آرتمياي پاراترانسژنيك در پرورش ميگو

PostDateIconيكشنبه ۲۱ شهریور ۱۳۸۹ ساعت ۰۸:۰۳ | PostAuthorIconنوشته شده توسط سيد مرتضي امامي | PDF | چاپ | نامه الکترونیک

بيولوژي دريا - پيشرفت تكنولوژي آرتمياي پاراترانسژنيك به منظور كنترل بيماري هاي عفوني در تكثير و پرورش ميگوي آب شور

روش ها و ابزار : اشريشياكولي ( Escherichia coli ) تراژني حاوي پروتئين هاي نشانگر فلوروسنت و سيانوباكتر Synechococcus bacillarus حاوي آنتي بادي تك زنجيره موش (DB3) به آرتميا فرانسيسكانا (Artemia franciscana) خورانده شد. انباشتگي و تمركز مولكول هاي نشانگر فراواني (مثل GPF و DS قرمز و DB3) پس از گذشت 10 ساعت از خورانده شدن اشريشياكولي در روده آرتميا مشاهده شد. نژادهاي دستكاري شده S. bacillarus حاوي DB3 به همراه فعاليت هاي آنتي بادي قابل ردگيري در روده آرتميا پس از 8 تا 10 ساعت از خورانده شدن از طريق ELISA انباشته شدند كه از لحاظ زماني با بيشترين تراكم S. bacillarus در روده آرتميا تطابق داشت.

نتيجه گيري : آرتمياي تغذيه كننده از باكتري ها يا جلبك هاي تراژني، پروتئين هاي نوتركيب را پس از 10 ساعت از آغاز فعاليت هاي بيولوژيكي انباشته كرد. ارسال همزمان چندين پروتئين نوتركيب به درون روده آرتميا نيز اين مطلب را اثبات نمود.

اهميت و تأثير اين پژوهش : معرفي مولكول هايي كه عوامل عفونت زا در پرورش ميگوي آب شور را مورد هدف قرار مي دهند از طريق ارگانيسم هايي كه معمولا مورد تغذيه ميگو قرار ميگيرند همچون آرتميا، ميتواند ابزار قدرتمندي را در روند جهاني افزايش توليدات غذايي فراهم نمايد.

 

تاريخ پذيرش مقاله : 30 ژوئن 2009

ترجمه شده از : www3.interscience.wiley.com/journal/122511221/abstract

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